基于单光源频域荧光寿命的水体溶解氧浓度检测(5)
为了更加直观表达响应时间以及探究两种传感器检测随时间变化的检测浓度值的关系,取正常放置1天的饱和空气溶氧水,向水中加入亚硫酸钠和催化剂六水氯化钴无氧水所制备的除氧试剂,快速置换传感器所处液体环境,将商业仪器和单光源检测方法分别得到的数据保存成图,归一化的结果如图8所示,直观看出二者具有较好的一致性,本文提出方法响应时间只有DOP1溶解氧分析仪的72%,响应时间更短,能够更快达到稳定状态.
图8 仪器数据对比图Fig.8 Comparison chart of instrument data
4 结论
本文提出了一种单光源的频域荧光寿命溶解氧检测方法.应用荧光淬灭法的原理,提出了降低光路结构和电路结构复杂性的单光源设计,对相频率法检测荧光寿命方法进行改进,使所提出的单光源检测方法同样可以避免相位偏移引发的误差问题,通过反演Stern-Volmer方程获取水体溶解氧浓度.同典型性的光学溶解氧分析仪DOP1进行对比分析,并设计变温实验探究温度同荧光寿命关系,实验结果表明,当溶解氧浓度在0~9 mg/L范围内,实验方法将检测精度提升至0.04 mg/L;衡量稳定性的检测标准偏差较对比仪器降低了36%,达到0.007 mg/L,稳定性更强;稳态时所需响应时间平均缩短了10 s,并且达到稳态时响应速度的提升均在18%以上;温度是荧光寿命的重要影响因素,同时会影响仪器的检测精度,因此需要设计温度检测电路对所得数据进行温度校准.该研究结果针对水体溶解氧浓度检测提供一种高标准的检测方法,能够适用于高精度、稳定性强以及快速响应的使用环境,并对荧光淬灭法在其它领域物质检测的应用具备一定的参考价值.
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